Principle
SMART-Seq uses reverse-transcriptase template switching to generate and amplify cDNA from single cells or small RNA inputs. Appropriate experiments provide more complete transcript coverage than end-tag strategies.
Applications
- Profile expression in single cells and low-input RNA samples
- Identify splice isoforms with suitable transcript coverage
- Compare transcription during development and differentiation
Method features
- Supports limited samples and precious cells
- Full-length coverage assists transcript and splicing analysis
- Input requirements and detection targets are assessed against sample conditions
Workflow
- 01
Assess single-cell or low-input RNA samples
- 02
Perform template-switching reverse transcription and cDNA amplification
- 03
Prepare, assess and sequence libraries
- 04
Quantify expression and analyze suitable transcript structures
This is a workflow overview. Experimental and analytical details depend on sample conditions and research objectives.