Principle
ATAC-Seq uses a transposase to cut accessible chromatin and insert sequencing adapters, producing a genome-wide accessibility profile. It can compare cells, treatments or tissues and integrate RNA-Seq or ChIP-Seq data to investigate gene regulation.
Applications
- Compare accessible regions between treated and control samples
- Prioritize candidate regulatory elements and analyze motifs
- Connect expression changes with chromatin states to develop regulatory hypotheses
Method features
- A streamlined workflow suitable for evaluating low-input projects
- Investigates chromatin accessibility genome-wide
- Supports integration with transcriptomics and protein-DNA interaction data
Workflow
- 01
Prepare and assess cells or nuclei
- 02
Perform Tn5 transposition and adapter insertion
- 03
Amplify, assess and sequence libraries
- 04
Identify accessible regions and compare regulatory signals
This is a workflow overview. Experimental and analytical details depend on sample conditions and research objectives.