Principle
MeDIP-Seq combines methylated-DNA immunoenrichment with sequencing to identify methylation-enriched genomic regions. Its main readout is regional signal, supporting pattern comparisons and candidate-region screening across samples.
Applications
- Compare methylated regions between tissues or treatments
- Investigate methylation patterns associated with disease
- Use regional annotation to prioritize candidate regulatory genes
Method features
- Uses antibody enrichment to investigate methylated regions
- Supports regional methylation screening
- Regional enrichment does not replace single-base methylation measurements
Workflow
- 01
Extract DNA and assess sample quality
- 02
Fragment DNA and immunoenrich methylated fragments
- 03
Prepare libraries and sequence
- 04
Identify methylated regions and compare enrichment
This is a workflow overview. Experimental and analytical details depend on sample conditions and research objectives.